Clean the lab benches with the bleach solution and remember to wash your hands before leaving the lab.
This activity will allow students to evaluate two patients with possible neurological symptoms. Students will come up with possible diagnosis and determine how to test for these diagnoses.
A patient and his wife come in to see you with a concern. The patient has a history of sickle cell disease in his family, but neither of his parents have exhibited any Gfp lab report. The wife is an immigrant from rural tropical Africa and has no idea if her family has any history of sickle cell disease.
However the area she is from has a high incidence of sickle cell anemia in the population. The couple has 2 children, ages 4, 8 and would like to have another.
The couple has come to you for advice on whether or not to have another child, and what to tell their children about the family medical history.
How is DNA analyzed and manipulated using restriction enzymes? Students compare the DNA fragments with the known restriction map of bacteria phage lambda. This activity demonstrates how DNA sequences can be mapped and characterized, such as in the Human Genome Project and how DNA is cut and arranged during genetic engineering.
What is genetic engineering, and how is this technique used? Students perform a genetic engineering experiment using bacterial transformation to introduce fluorescent genes into Escherichia coli E.
This activity helps students understand what genes do and how they can be manipulated by genetic engineering.
This activity will confirm that different genes introduced by transformation will result in different visible characteristics. This activity helps students to visualize regulation and relate this regulation to the lac operon system. Highly recommended for AP Biology. This activity allows the gene regulation concept to be presented in a more inquiry fashion.
Students will transform E. They will hypothesis why the cells did not glow and use PCR B to test their hypothesis. After the PCR the students will learn the regulation of this gene, and will induce the promoter to express the product. In this lab investigation students will learn about a technique called polymerase chain reaction PCR that allows us to examine a very small piece of DNA.
This gene codes for the GFP protein, an protein normally produced by jellyfish that is transformed into bacteria in a plasmid pGLO. This activity lends itself to be conducted inquiry style.Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in urbanagricultureinitiative.com can be excited by the nm laser line and is optimally detected at nm.
This shopping feature will continue to load items. In order to navigate out of this carousel please use your heading shortcut key to navigate to the next or previous heading. Green Fluorescent Protein I - 3 Background III: Hydrophobic Interaction Chromatography (HIC) Chromatography is a term that describes a huge and diverse set of methods for separating molecules based on their different properties.
Jun 23, · Soon afterward Ghia left my lab to do another lab rotation, and I asked Yuan Tu (Fig. 4), my technician at the time, to put GFP into C. elegans. Again the experiment succeeded, and for the first time we had GFP expressed in the touch receptor neurons of C. elegans (Fig.
6). This wet lab/tutorial consists of three separate parts, all dealing with the separation (purification) and analysis of proteins.
In your lab report, the first two parts should be presented separately according to the general guidelines for writing lab reports.
Sample Lab Report. The lab report is written in italics. Each section is repeated and in the second copy, hints and explanations are given.. Detection of p53 Protein in Normal Cells but Not Tumor Cells.